Atomic force microscopy can resolve structures on the nanometer scale. Scans as large as 90µm2 and less than 1µm2 can be collected on the MFP3D.
It has an extended Z range, permitting vertical displacements of up to 28µm. Both dry- and wet-mode scanning are available. A bioheater module facilitates live cell imaging for samples where temperature control is necessary.
Total internal reflection fluorescence (TIRF) microscopy is ideal for visualizing structures in the immediate vicinity (~100nm) of the coverslip including cell membrane structure and dynamics, and for resolving single molecules (e.g. DNA, cytoskeletal motility assays).
The system operates by reflecting a laser beam off of the coverslip in such a way that photons never hits the specimen directly; however the radiating evanescent wave is sufficient to illuminate fluorescent structures nearby. The result is superior signal-to-noise since structures outside the focal plane are never illuminated.
TIRF microscopy is also suitable for studying membrane dynamics in living cells since the laser does not induce damage.
The TIRF system consists of an Olympus IX81 with ZDC stage control fitted with 2 lasers (488 and 543nm) to accommodate a wide range of experimental demands. Phase and widefield fluorescence modalities are also available.
The AFM is also mounted on the microscope to obtain force and conductivity measurements as well as structural information on the nanometer scale.
Rates are hourly and are subject to change without further notice.
Valid through June 30, 2016.
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