Multiphoton Microscope

Zeiss LSM 510Meta/Multiphoton NLO

The Zeiss LSM NLO multiphoton microscope is equipped with a 25mW Argon laser (458, 477, 488, & 514nm), a 1mW green (543nm) and a 5mW red (633nm) HeNe laser, and a Ti;sapphire laser (Coherent Chameleon) tunable between 720-950nm.

The multiphoton is ideal for imaging very thick fluorescently labeled specimens with less photobleaching and attenuation than conventional confocal. Many live specimens can be imaged without causing damage.

In addition, the multiphoton is configured for second harmonic generation and confocal reflectance.

Rates & Fees: 

Rates are hourly and are subject to change without further notice.  Note: If using the confocal capability ONLY, the rate will be the same as for the Zeiss Confocal.

Valid through June 30, 2016.

  RPI Users External Not-for-Profit External Industry External Industry Partners
Standard $59 $96 $191 $172
Assisted $118 $191 $382 $344
Training $60 $97 $194 $175
Personnel Time $50 $81 $162 $146
Night-Flat Rate* $150 $240 $480 $360

* For the time between 8:00 pm until 8:00 am next morning (12 hours). This block of time is only for those experiments in which long acquisition times are needed in order to improve the quality of the data. 

Resources: 

LSM Image Browser: allows you to view your images in the comfort of your own home

Fluorescence dyes, filters, and objectives