|Cell and Molecular Overview|
Cell and Molecular Biology Core Facility
The Cell and Molecular Biology Core Facility provides state-of-the-art facilities to support experimental demands ranging from routine culture techniques to advanced cell transfection and cell sorting capabilities. Routine tissue culture needs are supported by two Biosafety Cabinets, a pair of CO2 incubators, refrigerators and freezers, environmental rooms, centrifuges, water baths, and inverted microscopes.
BD Sciences FACSAria flow cytometer is capable of identifying cells based on size, complexity, and up to 13 different fluorescent labels, and sorting them into separate test tubes or multiwell plates. The sample chamber includes temperature control to improve the long-term viability of the cells being sorted.
BD Sciences LSRII flow cytometer is capable of analyzing more fluorescent labels than the Aria, but lacks sorting capability. The system includes a High Throughput Sampler (HTS) option to handle 96- and 384-well microtiter plates that permits high-throughput screening of large samples.
GE Typhoon Trio+ is a confocal flatbed scanner with a 10mm lateral resolution scanner ideal for imaging fluorescently labeled blots, gels, multiwell trays, and even tissue sections. A storage phosphor can be used to detect radioactive signal from beta particles, X-rays, and gamma rays.
Roche LightCycler 480 RT-PCR The LightCycler 480 system utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes with exceptional reproducibility. Five excitation and six emission filters permit flexible experimental design. The LightCycler 480 System will meet the needs of a broad range of scientific applications in genomics research including microarray validation, gene-knockdown studies, and SNP analysis.
Amaxa Nucleofector uses a non-viral transfection technology to introduce nucleic acids (both DNA and RNA) into stem cells, primary cells, and cell lines that are difficult to transfect. Experiments involving transfection of siRNA or RNAi, transient or stable gene expression, and small-scale protein production are all possible.
Instructions for Online Scheduling:
Click on the 'Online Scheduling' link (above) to open the Calcium Calendar site.
Clicking the ‘Login’ button on the Calcium calendar site allows you to enter your RCS username and password.
Click ‘User Options’ on the following page, enter your current RCS password and email address, and click on 'Change Email Address'.
Next, send an email to Sergey Pryshchep (firstname.lastname@example.org) to be assigned the editing capabilities on the calendars you need to access.