Total internal reflection fluorescence (TIRF) microscopy is ideal for visualizing structures in the immediate vicinity (~100nm) of the coverslip including cell membrane structure and dynamics, and for resolving single molecules (e.g. DNA, cytoskeletal motility assays).
The system operates by bouncing a laser beam off of the coverslip so that photons never hits the specimen directly; however the radiating evanescent wave is sufficient to illuminate fluorescent structures nearby.
The result is superior signal-to-noise since structures outside the focal plane are never illuminated. TIRF microscopy is also suitable for studying membrane dynamics in living cells since the laser does not induce damage.
The TIRF system consists of an Olympus IX81 with ZDC stage control fitted with 3 lasers (488, 543 and 633nm) to accommodate a wide range of experimental demands. Phase contrast, DIC and widefield fluorescence modalities are also available. The Asylum MFP-3D atomic force microscope can also be mounted on the microscope to obtain force and conductivity measurements as well as structural information on the nanometer scale.
Rates are hourly and are subject to change without further notice.
Valid through July 31, 2018.
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