Olympus IX81 TIRF Microscope

Data produced by the Olympus IX81 TIRF Microscope

Total internal reflection fluorescence (TIRF) microscopy is ideal for visualizing structures in the immediate vicinity (~100nm) of the coverslip including cell membrane structure and dynamics, and for resolving single molecules (e.g. DNA, cytoskeletal motility assays).

The system operates by bouncing a laser beam off of the coverslip so that photons never hits the specimen directly; however the radiating evanescent wave is sufficient to illuminate fluorescent structures nearby.

The result is superior signal-to-noise since structures outside the focal plane are never illuminated. TIRF microscopy is also suitable for studying membrane dynamics in living cells since the laser does not induce damage.

Olympus IX81 in lab at Rensselaer
Olympus IX81 TIRF Microscope

The TIRF system consists of an Olympus IX81 with ZDC stage control fitted with 3 lasers (488, 543 and 633nm) to accommodate a wide range of experimental demands. Phase contrast, DIC and widefield fluorescence modalities are also available. The Asylum MFP-3D atomic force microscope can also be mounted on the microscope to obtain force and conductivity measurements as well as structural information on the nanometer scale.

Manufacturer: Olympus
Equipment Rates & Fees
  RPI Users External Nonprofit External Industry External Industry Partners
Standard $27 $44 $89 $80
Assisted $54 $89 $178 $160
Training $69 $114 $227 $204
  RPI Users External Nonprofit External Industry External Industry Partners
Personnel Time $60 $99 $197 $178

Valid through July 31, 2022. Rates are hourly unless specified otherwise and are subject to change without further notice.

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
Back to top