CBIS Students

Analytical Spectroscopic Analysis

Spectroscopic Methods in Biology and Biotechnology: A Survey with Applications

Spectroscopy: the study of how different wavelengths of light interact with matter is one of the most important tools available to Biology, Chemistry and Biotechnology to characterize and measure materials including: proteins, DNA, and small biologically active molecules. This course will present several spectroscopic methods and some of their current applications in the Biological Science, Biotechnology and clinical settings: today Absorbance in ELISA assays is used to determine whether people are mounting an immune response to COVID-19; Fluorescence is used to measure protein aggregation and fibril formation (important in Alzheirmer’s disease); Fourier-Transform Infrared is used in the recycling of plastics.


Sessions:

  • UV-Visible Absorbance: micro-volume and high-throughput methods; ELISA (enzyme-linked immunosorbent assay) tests
  • Fluorescence measurements with thioflavin T as a probe to monitor protein fibrils
  • Fourier-Transform Infrared using Attenuated Total Reflectance with application to recycling of polymer materials (plastics)

Contact:

Joel Morgan, PhD
Director Analytical Biochemistry Core Facility
Center for Biotechnology and Interdisciplinary Studies
Rensselaer Polytechnic Institute
e-mail: morgaj3@rpi.edu