Flow Cytometry

The Flow Cytometry Core Facility offers researchers powerful instruments to measure multiple physical characteristics of individual cells in suspension. The flow cytometry technology allows evaluation of multi-color immune-phenotyping, protein expression level, cell cycle analysis, apoptosis studies, and measures diverse cellular functions.

In addition, the Flow Cytometry Core Facility offers multi-color cell sorting to isolate specific cell types, as well as specific clones post-transfection for further cell culture or analysis. The Flow Cytometry Core Facility director provides the user with access to instrumentation, training and technical assistance, offers information on present methods and applications as well as on dyes selection and combination for multi-color panel, and other reagents.

Contact Information:
Sergey Pryshchep
Flow Cytometry Research Core Director
518-276-3456
pryshs@rpi.edu

 

FACSAria Flow Cytometer
FACSAria Flow Cytometer
The BD FACSAria cell sorter sets a new standard for high-performance flow cytometry. The 70- and 100-micron nozzle tip sizes are designed to accommodate most cell types. The flexibility of the BD FACSAria cell sorter provides the total solution for flow cytometry sorting.
LSRII Flow Cytometer
LSRII Flow Cytometer
Multi-fluorescence cell and particle analyzer. Configured with 405-nm, 488-nm, and 633-nm lasers, the LSRII has the ability to acquire up to 10 colors. The LSR II is also equipped with a plate loader option allowing automated throughput screening from 96 or 384 well plates. 

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.

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