Genomics

The Genomics Research Core Facility applies state-of-the-art next generation sequencing (NGS) technologies to interdisciplinary biomedical, biochemical and biological research. Adapting fast developing NGS tools, our key applications include whole genome resequencing for genome-wide polymorphism and mutation detection, transcriptome profiling for quantification of gene expression and discovery of novel transcripts, alternative splicing events and transcribed SNPs, sequencing of bisulfite-treated DNA for detection of methylated DNA, chromatin immunoprecipitation-sequencing (ChIP-seq) for genome-wide mapping of protein-DNA interactions, microbial metagenomic sequencing, small RNA sequencing, etc. For each application, we offer comprehensive services from high quality nucleic acid extraction, NGS library preparation and sequencing to bioinformatic data analysis, as well as consultation and training.

Image showing overview of the workflow from Library Prep to Sequencing to Data Analysis

The Genomics Core Facility houses:

Contact Information:
Yang Bai, Ph.D.
Genomics Research Core Director
518-276-4117
baiy4@rpi.edu

Sample Requirements

Only DNA and RNA samples are accepted in compliance with RPI Institutional Biosafety Committee protocols and policies.

Note: for dsDNA library, use the following formula to convert from ng/μl to nM: (concentration in ng/μl) / (600 g/mol x average library size in bp) x 106 = concentration in nM
Sample Type genomic DNA total RNA self-prepared library
Sample Purity (OD260/280) 1.8-2.0 1.8-2.0 1.8-2.0
Recommended RIN / ≥ 7.0 /
Recommended Quantity >5 µg, >100 ng/µl >3 µg, >100 ng/µl 10-50 nM, ≥ 10ul
Resuspension Buffer nuclease-free water, EB, or low TE buffer nuclease-free water nuclease-free water, EB, or low TE buffer
Shipment Method dry ice dry ice dry ice

Service Request

Step 1 - Request a project: 

  • For iLab users, click on https://rpi.corefacilities.org/account/login to log in the Genomics core iLab website. Under ‘Request Services’ tag, click ‘initiate request’ button to fill in project information step by step. 
  • For other users, please download and fill in the Project and Sample Request Form and email to baiy4@rpi.edu. New iLab account can be set up by request. 

Step 2 - Submit sample information:

  • For iLab users, templates of sample information will be provided in the system while you fill in your project request. Please download and fill in the template form, change file name (such as user_date) and upload back to iLab.    
  • For other users, if you submit samples for NGS library preparation and/or QC, please download and fill in the Template of Library Prep and email to baiy4@rpi.edu. If you submit self-prepared dsDNA libraries for sequencing, please download and fill in the Template of Sequence Self-prepared library and email to baiy4@rpi.edu. 

Step 3 - Deliver nucleic acid samples to the Genomics Core:

  • Pack according to the sample requirement and label tubes clearly. A sample list is recommended within the package.
  • For non-RPI users, if you order Illumina sequencing kit yourself, please directly ship it from the vendor to our core facility.
  • Mailing Address:

    Yang Bai
    Rensselaer Polytechnic Institute
    BioTech Bldg BT2434
    110 8th Street
    Troy NY, 12180-3590

Data storage

Sequencing and Analysis data will not be stored in the core server beyond 6 months.

Consultation

Monday and Thursday 4:00 pm -- 5:00 pm consultation is free of charge.  Other time consultation is by appointment only with hourly personnel fee.

Hours

Monday to Friday 9:00 am to 5:00 pm

Billing

Users will be required to provide billing information prior to requesting services. Users with multiple billing accounts should specify the account number to be charged upon sign-up. Both internal and external users will be billed on a monthly basis. Providing fraudulent and/or inaccurate billing information may result in the loss of use privileges.

Technology and Service:

image of code on a computer screen
Standard and custom library preparation services are available for diverse short-read and long-read sequencing strategies. Bioinformatics services include established and emerging pipelines for transcriptomic, genomic and epigenomic applications.
Illumina NextSeq 550
The NextSeq 550 offers flexible high-throughput sequencing capacity for exome, transcriptome and whole-genome sequencing of all sizes of genomes, as well as the ability to scan microarrays. It generates up to 120 Gb of sequence data overnight and allows a variety of output configurations for optimized target genome coverage.
Illumina iSeq 100
The iSeq 100 sequencing system is Illumina’s most accessible, cost-effective and rapid benchtop sequencer powered by CMOS technology and SBS chemistry. It generates 1.2 Gb of data per run.
Oxford Nanopore MinION at Rensselaer
The Oxford Nanopore MinION is a portable real-time sequencing device generating ultra-long reads from single DNA or RNA molecules without PCR amplification. Each MinION run generates 10-20 Gb of raw data with 75% of these reads passing quality control filters.
Sage Science BluePippin
The Sage Science BluePippin system is an automated DNA size selection tool for NGS applications, plus the capability of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA up to 50 kb.
Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer system is an established automated electrophoresis solution for the quality control of biomolecule samples. The system integrates an instrument, data processing software, reagents, and a microfluidic chip specific for DNA, RNA or protein analysis.
Invitrogen Qubit 4 Fluorometer
Qubit 4 fluorometer measures very low level of DNA, RNA, and protein concentration using as little as 1 μL of sample. It has higher sensitivity than UV absorbance–based quantification instruments, such as NanoDrop. RNA integrity values can also be quantified for total RNA samples.

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
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