Flow Cytometry Bootcamp

Characterization of Cell Therapy Products by Flow and Laser Force Cytometry

3 Day, bootcamp style, short course (50/50 hands on and lecture):

  • Where: Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies (CBIS)
  • When: TBD
  • Cost: $2200 ($1100 for NIIMBL members) - Price includes hotel, transportation, and all meals and evening events.

Who Should Attend

  • Industry Attendees: Employees with basic preliminary experience in Flow Cytometry but who want to move beyond a repetitive operator mind-set. (From How to Why).
  • Data Analytics/CMC: Employees who want to understand how their data is generated and how to connect experimental design with data quality and reliability.
  • Federal Regulators:  Employees that will be auditing/inspecting these facilities or making regulatory decisions based on this type of data.
  • Clinicians: Clinicians collecting and supplying samples for this type of research.

Course Content

Experimental Design and Planning Strategies

  • Dye Selection Strategies
  • Specific and Non-specific AB binding & Titration
  • Cocktail Staining Strategies
  • Appropriate Use of Beads and Calibrations
  • Phenotypic Characterization of T-cells (case study)

Hands on Equipment Operation & Troubleshooting

  • Control Strategies, Compensation Selection & troubleshooting
  • Cleaning, maintenance, clog prevention
  • Effective Bead use and Calibrations
  • Instrument troubleshooting

Data Analysis and Visualization

  • Scattering (FSC SSC)
  • Histograms
  • Multi-variant Analysis
  • Low Event Analysis

Laser Force Cytology

  • Basic Principals
  • Viral Infectivity Monitoring
  • CAR-T cell Applications
  • Gene Therapy Applications
  • Label Free Cell Sorting
  • Remote run & Data Analysis


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