Amaxa Nucleofector

The Amaxa nucleofector uses a novel non-viral transfection technology to introduce nucleic acids (both DNA and RNA) into stem cells, primary cells, and cell lines that are difficult to transfect. It employs a non-viral method based on an optimized combination of electrical parameters and cell-type specific solutions. Both DNA and RNA are efficiently transfected into both the cytoplasm and nucleus of cells. Thus, the Amaxa provides the ability to transfect even non-dividing cells, such as neurons and resting blood cells. In addition, the transfection efficiency is much higher than traditional methods can achieve. Experiments involving transfection of siRNA or RNAi, transient or stable gene expression, small-scale protein production are all possible.

The Amaxa is compatible with individual cuvettes as well as 96-well trays.

A great resource to determine optimal transfection settings for your favorite cells

Manufacturer: Lonza
Equipment Rates & Fees
 RPI UsersExternal NonprofitExternal IndustryExternal Industry Partners
 RPI UsersExternal NonprofitExternal IndustryExternal Industry Partners
Personnel Time$70$115$230$207

Valid through July 31, 2024. Rates are hourly unless specified otherwise and are subject to change without further notice.

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
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