Analytical Ultracentrifuge (AUC)
The Analytical Ultracentrifuge is used for two main types of measurements:
- Sedimentation Equilibrium measurements that are mostly intended to measure molecular weight and intermolecular association constants of the species in solution.
- Sedimentation Velocity experiments that are intended to measure sedimentation coefficients and hydrodynamic parameters of the species in solution.
Technical features
Our Beckman Proteomelab XL-I Analytical Ultracentrifuge is equipped with two types of detection:
- UV-Visible absorbance that can track species in solution that absorb light. Proteins can be tracked because they typically absorb at 280 nm in the ultraviolet.
- Rayleigh Interference Optics that track changes in refractive index, and can be used to follow species that do not absorb in the UV or visible ranges.
Up to 21 different sample/reference pairs can be studied in a single run. Samples can be recovered for additional analysis.
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Standard EQUILIBRIUM | $85 | $140 | $280 | $252 |
| Standard VELOCITY | $170 | $280 | $559 | $503 |
| Assisted | $170 | $280 | $559 | $503 |
| Training | $85 | $140 | $280 | $252 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $73 | $120 | $240 | $216 |
Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.
Required Acknowledgement and Authorship
Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.
- Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
- Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
- Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
