Circular Dichroism Spectrometer (CD) with Stopped-Flow Mixing Accessory
Jasco 815 Circular Dichroism Spectrophotometer and BioLogic SFM-400 Stopped-flow kinetics system.
The Circular Dichroism (CD) Spectrophotometer provides a relatively quick and easy way to evaluate the secondary structure of proteins in solution. The programmable thermoelectric sample temperature controller allows this measurement to be used to study thermal denaturation and evaluate thermal stability of proteins. The instrument can be configured for either CD or fluorescence measurements. In the CD configuration, the instrument can simultaneously record absorbance. The stopped-flow mixing capability makes it possible to study chemical denaturation of proteins and to follow other biological and chemical reactions on a millisecond time scale (primarily with fluorescence detection)
- Thermoelectric (“Peltier”) programmable sample temperature controller for thermal denaturation/melting experiments
- 4-syringe stopped-flow mixer allows for multi-step sequential mixing experiments with programmed delays
- Individual stepper-motor control of mixing syringes allows mixing ratios to be changed from computer without emptying syringes or switching out syringe barrels for experiments that require more than one mixing ratio.
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Standard | $20.41 | $34 | $67 | $60 |
| Assisted | $40.80 | $67 | $134 | $121 |
| Training | $85 | $140 | $280 | $252 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $73 | $120 | $240 | $216 |
Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.
Required Acknowledgement and Authorship
Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.
- Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
- Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
- Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
