Fluorometer with Polarization and Fluorescence Lifetime

The Fluorolog Tau-3 is a fluorometer that is configured for standard fluorescence spectroscopy as well as molecular dynamics measurements:

Standard fluorescence measurements: The Fluorolog Tau-3 can record excitation spectra and emission spectra by scanning one or the other of its two (excitation and emission) double monochromators. It can record changes in fluorescence over time (kinetics) by parking both the excitation and emission monochromators at fixed wavelengths. It can also record “synchronous fluorescence spectra” in which both monochromators are scanned, while keeping a constant offset between the excitation and the emission wavelengths.

Molecular dynamics capabilities: The instrument is equipped with polarizers that allow it to measure fluorescence polarization (or anisotropy). It can also measure fluorescence lifetimes using a frequency-domain method. In combination, the polarization and lifetime capabilities allow the Fluorolog Tau-3 to determine the speed of rotation of species in solution.

Manufacturer: Horiba (originally SPEX)


  • Fluorescence geometries: Right-angle and Front-face.
  • Sample stages:
    • Single holder for standard cuvettes with temperature control (thermo-electric “Peltier”) and magnetic stirring.
    • Two-cuvette carousel for fluorescence lifetime measurements, with temperature control by external circulating water bath.
    • Solid sample holder with rotational goniometer.
  • Light source: Xenon arc lamp
Equipment Rates & Fees
 RPI UsersExternal NonprofitExternal IndustryExternal Industry Partners
 RPI UsersExternal NonprofitExternal IndustryExternal Industry Partners
Personnel Time$70$115$230$207

Valid through July 31, 2024. Rates are hourly unless specified otherwise and are subject to change without further notice.

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