French Pressure Cell Disruptor

French Pressure Cell Disruptor

The “French  Pressure Cell” disrupts (“breaks”) the cells of microorganisms by elevating the cell suspension to a high pressure and then allowing it to escape back to ambient pressure through a small orifice. An alternative to the French Pressure Cell is the Microfluidizer, which operates on a similar principle but is suitable for much larger volumes.

The device was invented by an American scientist, Charles Stacey French, at  the Rockefeller Institute, and is named for him. The conditions of cell disruption can be carefully controlled by adjusting the applied pressure. It is most useful for small volumes of cell suspension (less than 35mL) or when the cells of microorganisms need to be broken in a very controlled way; for example, it can be used to prepare inside-out sub-bacterial vesicles from intact cells.

The Microbiology Core Facility has two “cells” for this device: A standard-volume cell that holds approximately 35mL, and and a“mini” cell that holds approximately 7mL.

Since the French Pressure Cell does not always shear the DNA released when  cells break, it is recommended to add DNAase (and Mg2+) prior to processing to avoid high viscosity conditions

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Personnel Time $63 $104 $207 $187

Valid through July 31, 2022. Rates are hourly unless specified otherwise and are subject to change without further notice.

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