Illumina iSeq 100

The iSeq 100 sequencing system is Illumina’s most accessible, cost-effective and rapid benchtop sequencer powered by CMOS technology and SBS chemistry. By generating 1.2 Gb of data per run in only 17.5 hours, the iSeq 100 System delivers the high resolution and analytical sensitivity obtained on other Illumina sequencing platforms. Compared to qPCR and Sanger sequencing, the iSeq 100 System allows greater statistical confidence for calling variants or low-frequency alleles. This is the ideal platform for microbial and viral whole-genome sequencing, targeted gene sequencing such as small cancer panels, targeted gene expression profiling and miRNA & small RNA analysis.

Manufacturer: Illumina
Sequencing Kit Catalog # Read Length (bp) SE Read # PE Read # Output Data
iSeq 100 i1 Reagent 20021533 2 × 150, 1 x 300 4 million 8 million 1.2 Gb
Equipment Rates & Fees
Customers are encouraged to purchase kits on their own and arrange adequate shipping to the core facility for sequencing.
ServiceUnitRPI UserExternal NonprofitExternal IndustryExternal Industry Partner
Sequencingper run$69$114$227$204
 RPI UsersExternal NonprofitExternal IndustryExternal Industry Partners
Personnel Time$73$120$240$216

Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
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