Illumina NextSeq 550

The NextSeq 550 is a flexible and efficient platform that offers powerful high-throughput sequencing capabilities. Designed to finish each sequencing task independently and fast (within 30 hours), it is the newest high-throughput desktop sequencer to offer exome, transcriptome and whole-genome sequencing for all sizes of genomes. The flexibility of this platform allows a variety of flow cell configurations for optimized depth of coverage. Its sequencing capacity is up to 800 million paired-end reads and up to 400 million single reads per run.

Manufacturer: Illumina
Sequencing Kit Catalog # Read Length (bp) SE Read # PE Read # Output Data
NextSeq 500/550 High Output Kit (300 Cycles) 20024908 2 x 150, 1 x 300 400 million 800 million 100-120 Gb
NextSeq 500/550 High Output Kit (150 Cycles) 20024907 2 x75, 1 x 150 400 million 800 million 50-60 Gb
NextSeq 500/550 Mid Output Kit (300 Cycles) 20024905 2 x 150, 1 x 300 130 million 260 million 39 Gb
NextSeq 500/550 High Output Kit (75 Cycles) 20024906 1x75 400 million 800 million 25-30Gb
NextSeq 500/550 Mid Output Kit (150 Cycles) 20024904 2 x75, 1 x 150 130 million 260 million 16.25-19.5 Gb
Equipment Rates & Fees
The sequencing reagent kit price varies based on read length (cycle number) and output mode. Please check the Kit Choice Table above for details and a side-by-side comparison. Customers are encouraged to purchase kits on their own and arrange adequate shipping to the core facility for sequencing.
ServiceUnitRPI UserExternal NonprofitExternal IndustryExternal Industry Partner
Sequencingper run$204$336$671$604
 RPI UsersExternal NonprofitExternal IndustryExternal Industry Partners
Personnel Time$70$115$230$207

Valid through July 31, 2024. Rates are hourly unless specified otherwise and are subject to change without further notice.

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
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