Illumina NextSeq 550
The NextSeq 550 is a flexible and efficient platform that offers powerful high-throughput sequencing capabilities. Designed to finish each sequencing task independently and fast (within 30 hours), it is the newest high-throughput desktop sequencer to offer exome, transcriptome and whole-genome sequencing for all sizes of genomes. The flexibility of this platform allows a variety of flow cell configurations for optimized depth of coverage. Its sequencing capacity is up to 800 million paired-end reads and up to 400 million single reads per run.
| Sequencing Kit | Catalog # | Read Length (bp) | SE Read # | PE Read # | Output Data |
|---|---|---|---|---|---|
| NextSeq 500/550 High Output Kit (300 Cycles) | 20024908 | 2 x 150, 1 x 300 | 400 million | 800 million | 100-120 Gb |
| NextSeq 500/550 High Output Kit (150 Cycles) | 20024907 | 2 x75, 1 x 150 | 400 million | 800 million | 50-60 Gb |
| NextSeq 500/550 Mid Output Kit (300 Cycles) | 20024905 | 2 x 150, 1 x 300 | 130 million | 260 million | 39 Gb |
| NextSeq 500/550 High Output Kit (75 Cycles) | 20024906 | 1x75 | 400 million | 800 million | 25-30Gb |
| NextSeq 500/550 Mid Output Kit (150 Cycles) | 20024904 | 2 x75, 1 x 150 | 130 million | 260 million | 16.25-19.5 Gb |
| Service | Unit | RPI User | External Nonprofit | External Industry | External Industry Partner |
|---|---|---|---|---|---|
| Sequencing | per run | $210 | $345 | $691 | $622 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $73 | $120 | $240 | $216 |
Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.
Required Acknowledgement and Authorship
Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.
- Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
- Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
- Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
