Isothermal Titration Calorimeter
Isothermal Titration Calorimetry allows a simultaneous determination of all binding parameters (equilibrium constant, enthalpy of binding and stoichiometry of the interaction) in a single experiment - information unobtainable from more limited binding assays.
- Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
- Assessment of the effect of molecular structure changes on binding mechanisms.
- Assessment of biological activity.
- The range of binding constants is broad: K from sub-millimolar to nanomolar (102 to 109 M-1).
- It is a true in-solution technique: No labeling or immobilization is required.
- Active cell volume ~ 1.4 ml.
- Non-reactive Hastelloy® cells for excellent chemical resistance.
- Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance.
- Precision liquid delivery system for accurate and reproducible injections.
- Operating temperature range of 2ºC to 80ºC.
- Peltier controlled for rapid temperature equilibration.
- Includes ThermoVac® sample preparation and cleaning device.
- Includes Single Injection Method and ITC-Expert software.
Equipment Rates & Fees
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Approved | $20.41 | $34 | $67 | $60 |
| Assisted | $40.82 | $67 | $134 | $121 |
| Training | $85 | $140 | $280 | $252 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $73 | $120 | $240 | $216 |
Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.
Required Acknowledgement and Authorship
Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.
- Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
- Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
- Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
