Laser Scanning Confocal Microscope - Zeiss LSM 510 META l
Zeiss LSM 510 META l
Laser scanning confocal microscope is ideal for imaging thick fluorescently labeled specimens thanks to the ability to collect thin optical sections with minimal background fluorescence from out-of-focus structures. The Zeiss LSM is equipped with a META detector that can separate numerous fluorescent labels with overlapping emissions.
The Zeiss LSM 510 laser scanning confocal microscope is equipped with a 30mW 405nm-diode, a 25mW Argon laser (458, 477, 488, & 514nm), a 1mW green (543nm) and a 5mW red (633nm) HeNe laser.
Confocal microscopy is ideal for imaging thick fluorescently labeled specimens thanks to the ability to collect thin optical sections with minimal background fluorescence from out-of-focus structures.
The Zeiss LSM is equipped with a Meta detector that can capture emissions ranging from 411-754nm, in 10.7nm bins across 32 channels. Using the Meta, multiple fluorescent labels with overlapping emissions can be separated using linear unmixing algorithms.
LSM Image Browser -- allows you to view your images in the comfort of your own home
Objectives
10x 0.3 N.A. (Plan-Neofluar) NO immersion
- working distance = 5.5 mm
25x o.8 N.A. (Plan-Neofluar, 1mm DIC) variable immersion
- working distance = 0.21 mm
- correction collar for different immersion media
40x 0.6 N.A. (LD Plan-Neofluar) NO immersion
- working distance = 2.9 mm @ coverglass 0.75
- correction collar for plastic & coverslip thickness
40x 1.2 N.A. (C-Apochromat, UV-VIS-IR) Water immersion, DIC
- working distance = 0.28 mm @ #1.5 coverglass
- correction collar for coverslip thickness
63x 1.4 N.A. (Plan-Apochromat) Oil immersion, DIC
- working distance = 0.19 mm
100x 1.45 N.A. (a Plan-Fluar) Oil Immersion, DIC
- working distance = 0.11 mm
Lasers
405 nm diode (violet)
-
Suitable for, for example, DAPI, Hoechst, Alexa405, Pacific Blue
458, 488, 514 nm argon laser (blue)
- Suitable for, for example, CFP, GFP, YFP, FITC, Alexa488, Cy2
543 nm HeNe laser (green)
- Suitable for, for example, mCherry, RFP, rhodamine, texas red, Alexa546, Alexa568, Cy3
633 nm HeNe laser (red)
- Suitable for, for example, IRP, Cy5, Alexa633, Alexa647
Filters
Ch2
- LP 420
- LP 475
- LP 505
- BP 420-480
- BP 470-500
- BP 475-525
- BP 505-530
Ch3
- LP 505
- LP 560
- LP 650
- BP 510/20
- BP 505-530
- BP 530-600
- BP 560-615
ChS
- 411.3-753.7 nm
Additional Features
- Axiovert 200 Inverted Microscope
- Zeiss AIM software – includes time lapse, photobleaching, FRAP, FRET capability
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Standard | $48 | $79 | $158 | $142 |
| Assisted | $96 | $158 | $316 | $284 |
| Training | $85 | $140 | $280 | $252 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $73 | $120 | $240 | $216 |
Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.
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