Laser Scanning Confocal Microscope - Zeiss LSM 510 META l

Example of image produced by the Zeiss LSM 510 META

Zeiss LSM 510 META l

Laser scanning confocal microscope is ideal for imaging thick fluorescently labeled specimens thanks to the ability to collect thin optical sections with minimal background fluorescence from out-of-focus structures. The Zeiss LSM is equipped with a META detector that can separate numerous fluorescent labels with overlapping emissions.

Zeiss LSM 510 META 1The Zeiss LSM 510 laser scanning confocal microscope is equipped with a 30mW 405nm-diode, a 25mW Argon laser (458, 477, 488, & 514nm), a 1mW green (543nm) and a 5mW red (633nm) HeNe laser.

Confocal microscopy is ideal for imaging thick fluorescently labeled specimens thanks to the ability to collect thin optical sections with minimal background fluorescence from out-of-focus structures.

The Zeiss LSM is equipped with a Meta detector that can capture emissions ranging from 411-754nm, in 10.7nm bins across 32 channels. Using the Meta, multiple fluorescent labels with overlapping emissions can be separated using linear unmixing algorithms.

Manufacturer: Zeiss

LSM Image Browser -- allows you to view your images in the comfort of your own home

Fluorescence dyes, filters, and objectives

Objectives

10x 0.3 N.A. (Plan-Neofluar) NO immersion

  • working distance = 5.5 mm

25x o.8 N.A. (Plan-Neofluar, 1mm DIC) variable immersion

  • working distance = 0.21 mm
  • correction collar for different immersion media

40x 0.6 N.A. (LD Plan-Neofluar) NO immersion

  • working distance = 2.9 mm @ coverglass 0.75
  • correction collar for plastic & coverslip thickness

40x 1.2 N.A. (C-Apochromat, UV-VIS-IR) Water immersion, DIC

  • working distance = 0.28 mm @ #1.5 coverglass
  • correction collar for coverslip thickness

63x 1.4 N.A. (Plan-Apochromat) Oil immersion, DIC

  • working distance = 0.19 mm

100x 1.45 N.A. (a Plan-Fluar) Oil Immersion, DIC

  • working distance = 0.11 mm

Lasers

405 nm diode (violet)

  • Suitable for, for example, DAPI, Hoechst, Alexa405, Pacific Blue

458, 488, 514 nm argon laser (blue)

  • Suitable for, for example, CFP, GFP, YFP, FITC, Alexa488, Cy2

543 nm HeNe laser (green)

  • Suitable for, for example, mCherry, RFP, rhodamine, texas red, Alexa546, Alexa568, Cy3

633 nm HeNe laser (red)

  • Suitable for, for example, IRP, Cy5, Alexa633, Alexa647

Filters

Ch2

  • LP 420
  • LP 475
  • LP 505
  • BP 420-480
  • BP 470-500
  • BP 475-525
  • BP 505-530

Ch3

  • LP 505
  • LP 560
  • LP 650
  • BP 510/20
  • BP 505-530
  • BP 530-600
  • BP 560-615

ChS

  • 411.3-753.7 nm

Additional Features

  • Axiovert 200 Inverted Microscope
  • Zeiss AIM software – includes time lapse, photobleaching, FRAP, FRET capability
Equipment Rates & Fees
  RPI Users External Nonprofit External Industry External Industry Partners
Standard $42 $69 $138 $124
Assisted $84 $138 $276 $249
Training $69 $114 $227 $204
  RPI Users External Nonprofit External Industry External Industry Partners
Personnel Time $60 $99 $197 $178

Valid through July 31, 2022. Rates are hourly unless specified otherwise and are subject to change without further notice.

Required Acknowledgement and Authorship

Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.

  • Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
  • Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
  • Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
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