CBIS Facilities
Research Faculty at CBIS

NextSeq 550 sequencer

The NextSeq 550 is a flexible and efficient platform that offers powerful high-throughput sequencing capabilities. Designed to finish each sequencing task independently and fast (within 30 hours), it is the newest high-throughput desktop sequencer to offer exome, transcriptome and whole-genome sequencing for all sizes of genomes. The flexibility of this platform allows a variety of flow cell configurations for optimized depth of coverage. Its sequencing capacity is up to 800 million paired-end reads and up to 400 million single reads per run.

Sequencing Kit Catalog # Read Length (bp) SE Read # PE Read # Output Data
NextSeq 500/550 High Output Kit (300 Cycles) 20024908 2 x 150, 1 x 300 400 million 800 million 100-120 Gb
NextSeq 500/550 High Output Kit (150 Cycles) 20024907 2 x75, 1 x 150 400 million 800 million 50-60 Gb
NextSeq 500/550 Mid Output Kit (300 Cycles) 20024905 2 x 150, 1 x 300 130 million 260 million 39 Gb
NextSeq 500/550 High Output Kit (75 Cycles) 20024906 1x75 400 million 800 million 25-30Gb
NextSeq 500/550 Mid Output Kit (150 Cycles) 20024904 2 x75, 1 x 150 130 million 260 million 16.25-19.5 Gb
Rates & Fees: 

The sequencing reagent kit price varies based on read length (cycle number) and output mode. Please check the Kit Choice Table above for details and a side-by-side comparison. Customers are encouraged to purchase kits on their own and arrange adequet shipping to the core facility for sequencing.

Valid through July 31, 2019

Service Unit RPI User ($) External Nonprofit ($) External Industry ($) External Ind Partner ($)
Sequencing Per run 104 168 337 303