Roche LightCycler 480 RT-PCR and Step-One Plus real-time PCR
The LightCycler 480 system utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes with exceptional reproducibility.
Five excitation and six emission filters permit flexible experimental design, using labels including SYBR Green I, hydrolysis probes, HypProbe probes, SimpleProbe probes, Molecular Beacons, and others.
The LightCycler 480 System will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.
StepOnePlus Real-Time PCR System
96-well Real-Time PCR instrument perfect for both first-time and experienced users. Utilizing robust LED based 4-color optical recording, it is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.
Features:
- Advanced software and instrumentation for performing a wide array of genomic assays
- Sensitive 4-color optical LED recording system
- Simple and flexible instrument set-up and usage
Specifications:
- Format: 96-well plate, 0.1 ml tubes, 8-tube strips
- Optics: 4 emission filters, blue LED excitation source, photodiode
- Capacity: 96 x 0.1 ml tubes, 1 x 96-well plate, 12 x 8-tube strips
- Run time: <40 min/run (Fast Mode), <2 hrs/run (Standard Mode)
- Sensitivity: 1 copy
- Dynamic range: Linear Dynamic Range greater than 9 log units (detection)
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Standard | $27 | $44 | $89 | $80 |
| Assisted | $54 | $89 | $178 | $160 |
| Training | $89 | $146 | $293 | $264 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $77 | $127 | $253 | $228 |
Effective August 1, 2025 through July 31, 2026. Rates are hourly unless specified otherwise and are subject to change without further notice.
Required Acknowledgement and Authorship
Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.
- Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
- Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
- Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
