Thermo Scientific LTQ ORBITRAP XL
The LTQ Orbitrap XL™ is ideal for high-resolution (up to 100,000) accurate mass measurements (~2 ppm) and structural characterization of individual compounds and complex mixtures with very high sensitivity. It provides a wide dynamic range, outstanding mass accuracy,and fast cycle times for MS/MS experiments.
The instrument is used for the characterization of intact proteins, top-down and bottom up proteomics, for the analyses of carbohydrates, oligonucleotides, estrogens, alkaloids, and glycerides.
Micro-or nano-flow Agilent 1200 HPLC front-end or direct injection ESI experiments
- High resolution (up to 100,000) and accurate mass measurements (up to 2 ppm)
- Extended mass range (m/z up to 4,000)
- Precursor ionisolation and fragmentation in the linear ion trap with high resolution and accurate mass MS/MS and MSn(n=2-10) data acquisition in the Orbitrap
- HCD dissociation in the C-trap
- Data dependent data acquisition
- XCalibur software for instrument control and data analysis
- Protein Discoverer software for protein/peptide identification
- "Non-anticoagulant low molecular weight heparins for pharmaceutical applications", Y. Ouyang, Y. Yu, F. Zhang, J. Chen, X. Han, K. Xia, Y. Yao, Z. Zhang, R. J. Linhardt, Journal of Medicinal Chemistry, 62, 1067-1073, 2019.
- "Production of pyranoanthocyanins using Escherichia coli co-cultures", Akdemir H., Silva, A., Zha J., Zagorevski D. V.; Koffas M., Metabolic egineering 55, 290-298, 2019.
- "Structural and functional components of the skate sensory organ ampullae of Lorenzini", X. Zhang, K. Xia, L. Lin, F. Zhang, Y. Yu, K. St.Ange, X. Han, E. Edsinger, J. J. Sohn, R. J. Linhardt, ACS Chemical Biology, 13, 1677–1685, 2018.
- "Core sequence of PAPf39 amyloid fibrils and mechanism of pH-dependent fibril formation: the role of monomer conformation", French K.C., Makhatadze G.I., Biochemistry. 51, 10127-36, 2012.
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Standard | $59 | $97 | $194 | $175 |
| Assisted | $118 | $194 | $388 | $349 |
| Training | $80 | $132 | $263 | $237 |
| nano-UPLC MS | $69 | $114 | $227 | $204 |
| RPI Users | External Nonprofit | External Industry | External Industry Partners | |
|---|---|---|---|---|
| Personnel Time | $73 | $120 | $240 | $216 |
Valid through July 31, 2025. Rates are hourly unless specified otherwise and are subject to change without further notice.
Required Acknowledgement and Authorship
Please acknowledge the CBIS Core Facilities in all publications and grant applications where our equipment and/or personnel have facilitated the work. These acknowledgements are very important because documenting our contributions helps to ensure that the resources of the Core Facilities are sustainable.
- Equipment: If you used Core Facility equipment, please note this in the Materials and Methods. e.g., Thermogravimetric analysis was carried out using a TA Instruments TGA-Q50 (Rensselaer CBIS Analytical Biochemistry Core Facility).
- Personnel: Please consider including CBIS personnel as co-authors on your publications when they have made a significant intellectual contribution to the research. Include CBIS Core Facility directors or staff as co-PI or co-investigators in grant applications when they provide a significant contribution to the grant proposal and scientific/intellectual leadership for the proposed work. Please follow these guidelines: ABRF Recommended Guidelines for Authorship on Manuscripts. Also, our Core Facility personnel always appreciate when they are mentioned in the Acknowledgements section of publications.
- Required Funding Authorization Form: Rensselaer researchers must fill out the CBIS Cores Authorization Form (PDF) to use the CBIS Core Facilities.
