The Cell and Molecular Biology Core Facility provides state-of-the-art facilities to support experimental demands ranging from routine culture techniques to advanced cell transfection and cell sorting capabilities. Routine tissue culture needs are supported by two Biosafety Cabinets, a pair of CO2 incubators, refrigerators and freezers, environmental rooms, centrifuges, water baths, and inverted microscopes.
- GE Typhoon Trio+ Flatbed Scanner for imaging fluorescently labeled blots, gels, multiwell trays, and even tissue sections with up to 10μm lateral resolution. A phosphor screen can be used to retain signal from beta particles, X-rays, and gamma rays.
- Tissue Culture Facility includes biosafety cabinets, refrigerators, water baths, centrifuges, and incubators for routine tissue culturing needs.
- The Amaxa Nucleofector for high-efficiency, non-viral transfection to deliver DNA and RNA to both cytoplasm and nucleus of cells and bacteria, including difficult primary cell lines.
- Roche LightCycler 480 is a high-throughput, real-time PCR for a broad range of genomics applications, including:
- Array validation, gene knockdown studies, SNP analysis, and genotyping.
- Flow Cytometry Facilities includes a BD FACS Aria and BD-LSRII for high-throughput cell counting and sorting of up to four different cell types based on size and 10+ fluorescent markers.