CBIS Facilities

Genomics

The Genomics Research core is located on the second floor in the Center for Biotechnology and Interdisciplinary Studies.

The facilities offer NGS library preparation, sequencing and data analysis services, featuring Illumina NextSeq 550 sequencer, iSeq 100 sequencer, Agilent 2100 Bioanalyzer, Qubit 4 flurometer, Bio-Rad thermal cycler, Bio-Rad nucleic acid electrophoresis system and Dell 48-core server ‘double-helix’. Roche LightCycler and Bio-Rad ChemiDoc from CMB core are utilized for qPCR amplification and nucleic acid imaging.

RPI Genomics Flowchart:

Contact Information: 

Yang Bai, Ph.D.
Genomics Research Core Director
518-276-4117
baiy4@rpi.edu

Mailing address:
Yang Bai
Rensselaer Polytechnic Institute
BioTech Bldg BT2434
110 8th Street
Troy NY, 12180-3590

Technology available through other core facilities: 
Policy for Use: 

Sample Requirements

Only DNA and RNA samples are accepted in compliance with RPI Institutional Biosafety Committee protocols and policies

Sample Type genomic DNA total RNA self-prepared library
Sample Purity (OD260/280) 1.8-2.0 1.8-2.0 1.8-2.0
Recommended RIN / ≥ 7.0 /
Recommended Quantity >5 µg, >100 ng/µl >3 µg, >100 ng/µl 10-50 nM, ≥ 10ul
Resuspension Buffer nuclease-free water, EB, or low TE buffer nuclease-free water nuclease-free water, EB, or low TE buffer
Shipment Method dry ice dry ice dry ice

Note: for dsDNA library, use the following formula to convert from ng/μl to nM:
(concentration in ng/μl) / (600 g/mol x average library size in bp) x 106 = concentration in nM

Service Request

Step 1 - Request a project: 

Step 2 - Submit sample information:

  • For iLab users, templates of sample information will be provided in the system while you fill in your project request. Please download and fill in the template form, change file name (such as user_date) and upload back to iLab.    
  • For other users, if you submit samples for NGS library preparation and/or QC, please download and fill in the Template of Library Prep and email to baiy4@rpi.edu. If you submit self-prepared dsDNA libraries for sequencing, please download and fill in the Template of Sequence Self-prepared library and email to baiy4@rpi.edu

Step 3 - Deliver nucleic acid samples to the Genomics Core:

  • Pack according to the sample requirement and label tubes clearly. A sample list is recommended within the package.
  • For non-RPI users, if you order Illumina sequencing kit yourself, please directly ship it from the vendor to our core facility.
  • Mailing Address:

    Yang Bai
    Rensselaer Polytechnic Institute
    BioTech Bldg BT2434
    110 8th Street
    Troy NY, 12180-3590

Data storage 

Raw sequencing data will be stored in the core server for three months free of charge. Requesting further storage, please contact the core director (Yang Bai, baiy4@rpi.edu).

Consultation

Monday and Thursday 4:00pm-5:00pm consultation is free of charge.  
Other time consultation is by appointment only with hourly personnel fee.

Hours

Monday to Friday 9:00AM to 5:00PM

Billing

Users will be required to provide billing information prior to requesting services. Users with multiple billing accounts should specify the account number to be charged upon sign-up. Both internal and external users will be billed on a monthly basis. Providing fraudulent and/or inaccurate billing information may result in the loss of use privileges.